thank you for taking the time to look at my post. I was wondering if there is anyone who could help me to calculate the molecular weight of a protein from the protein sequence, ideally manually. I heard that for a rough estimate the following can be used:
No. of amino acids * 110 (i.e. the average mol wt of an amino acid) - No of amino acids * 18 (i.e mol wt of water)
I am also aware that there are numerous web servers available to do this, however, I have many hundreds of thousands of sequences and I want to incorporate this into my programs which performs other calculations.
If you cannot help with this, could you suggest an accurate stand alone program that I can use via a commandline call to do this?
Many thanks for your time.