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new to BioEdit- help please

new to BioEdit- help please - Bioinformatics

new to BioEdit- help please - Have questions about bioinformatic tools or databases? Post questions here. Discuss and post interesting bioinformatics information.


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Old 01-06-2012, 05:17 PM
Pipette Filler
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Default new to BioEdit- help please



Hello, all.
I am very new to software BioEdit. So please bare with my basic questions.
I want to use bioedit to compare pairwise similarity between two or more genes.
After selecting both genes, which tab shall i go to get the % similarity data i am looking for?

Is it under sequence tab--pairwise alignment?
Or I have to calculate a similarity matrix first?

please help
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Old 01-06-2012, 05:23 PM
Pipette Filler
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Default Re: new to BioEdit- help please

and under sequence tab-pairwise alignment-calculate silimlarity/identity for two sequence
gives me a result:

Sequence 1: A
Sequence 2: D

Identities: 0.2212349
what does it mean, does it mean that only 22% of the sequence are the same?

The paper I read, they published similarity percentage data, between A and D, they are about 72% similar to each other, so i guess the identities: 0.2212349 does not make sence? please help?!! I am desperate. noone in my lab is using this software, there are soo many things I need to teach myself.
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