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BLAST small peptide
I have make a novel antibody against my study protein and I have some problems with it. I believe that it can recognize another cellular proteins, but when I make a blast I did not have results, because the peptide only alignment with my protein.
I have probe different blast programs but they don't work if the peptide is smaller than 10 aa.
Re: BLAST small peptide
Well, different blast tools do different things. You need to single out on one or two tools that can do the work for you.
(Eg... If I was looking for ESTs, I would use tblast ot tblastx only)
The reason you did not have any results is maybe that you did not get a decent enough bit score.
|blast , peptide , small|
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