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BLAST small peptide

BLAST small peptide - Bioinformatics

BLAST small peptide - Have questions about bioinformatic tools or databases? Post questions here. Discuss and post interesting bioinformatics information.


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Old 10-25-2010, 03:23 PM
Pipette Filler
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Question BLAST small peptide



Hi!

I have make a novel antibody against my study protein and I have some problems with it. I believe that it can recognize another cellular proteins, but when I make a blast I did not have results, because the peptide only alignment with my protein.

I have probe different blast programs but they don't work if the peptide is smaller than 10 aa.

Some idea??

Thanks.

María
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Old 11-06-2010, 09:01 PM
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Default Re: BLAST small peptide

Well, different blast tools do different things. You need to single out on one or two tools that can do the work for you.
(Eg... If I was looking for ESTs, I would use tblast ot tblastx only)

The reason you did not have any results is maybe that you did not get a decent enough bit score.
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