Using Bioedit for generating a sequence.

[nettle]

Hello everyone,

I usaully use Bioedit for editing DNA sequences for my project on phylogenetic analysis. This time I had a problem and can't solve it.
Its just that I got a gene sequenced using three primers, two were forward primers and third one reverse. Now, I dont understand how to combine all three obtained sequences of the target gene using Bioedit?

Kindly help.

[Warthaug]

I don't have bioedit in front of me, but what you need to do is take the sequence from the reverse primer and use the "reverse-complement" command (it is under one of the drop-down menus).

You can then use the "sliding ends" alignment tool (also under a drop-down menu) to align the three sequences.

Bryan

[nettle]

Thanks for the help Bryan. It did work out.

Nettle.