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Using Bioedit for generating a sequence.

Using Bioedit for generating a sequence. - Bioinformatics

Using Bioedit for generating a sequence. - Have questions about bioinformatic tools or databases? Post questions here. Discuss and post interesting bioinformatics information.


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  #1  
Old 07-18-2010, 11:07 AM
Pipette Filler
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Default Using Bioedit for generating a sequence.



Hello everyone,

I usaully use Bioedit for editing DNA sequences for my project on phylogenetic analysis. This time I had a problem and can't solve it.
Its just that I got a gene sequenced using three primers, two were forward primers and third one reverse. Now, I dont understand how to combine all three obtained sequences of the target gene using Bioedit?

Kindly help.
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Old 07-21-2010, 12:52 PM
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Default Re: Using Bioedit for generating a sequence.

I don't have bioedit in front of me, but what you need to do is take the sequence from the reverse primer and use the "reverse-complement" command (it is under one of the drop-down menus).

You can then use the "sliding ends" alignment tool (also under a drop-down menu) to align the three sequences.

Bryan
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Old 07-25-2010, 06:56 AM
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Default Re: Using Bioedit for generating a sequence.

Thanks for the help Bryan. It did work out.

Nettle.
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