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Bioinformatics coursework help!

Bioinformatics coursework help! - Bioinformatics

Bioinformatics coursework help! - Have questions about bioinformatic tools or databases? Post questions here. Discuss and post interesting bioinformatics information.


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Old 04-27-2010, 10:58 AM
Pipette Filler
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Default Bioinformatics coursework help!



Hey,

I have a piece of coursework that I'm completely stuck on. I've been given an unknown DNA sequence of just under 7kb and been told to analyse it and write a paper on it.

The basic idea is to attempt to work out what organism it's from and what it codes for.

In terms of structure I've ran it through ORF finder, GeneMark and Glimmer and have found what appears to be 5 coding regions, 3 for a radial SAM domain, 1 for an aminotransferase and 1 for a nucleosidase/phosphorylase...

...and then I get a bit stuck.

We haven't really been given much direction on what to include but I need to think about what the organism is, what it is related to, what genes it has, what those genes do, how they are organized and what all of this tells me about the organism and the environment?

Any help would be greatly appreciated.
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Old 07-14-2010, 10:09 AM
Pipette Filler
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Default Re: Bioinformatics coursework help!

Search for similiarities to the sequence with BLAST. Try the complete sequence or parts of it. Or search on NCBI instead of BLAST. You'll probably get your information about organism, maybe phylogenetics and the functional parts of your sequence. If you stuck completely send a part of the sequence (about 100-200bp should be enough) and I'll see if I get the information you want.
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