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Tm calculation using thermodynamic concept for Degenerate Primers?

Tm calculation using thermodynamic concept for Degenerate Primers? - Bioinformatics

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Old 04-14-2010, 06:12 PM
Pipette Filler
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Default Tm calculation using thermodynamic concept for Degenerate Primers?



Does anyone know how to compute the melting temperature for Degenerate Primers following the thermodynamic nearest neighbor model? or how it works? what I found so far is Tm calculations for primers with A,C,G, or T only, but haven't came across anything that can help me explain the way it works for degenerate primers, so I can implement that in my perl code.

any help please? Thanks!
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