DNA sequences....what's next?

[dym27]

Hi I'm a Marine biology student and I have the FWD and the Reverse sequences of a sea turtle gene I'm studying,,,,,

I need to join them so they look as a double DNA strand,,,please can anyone tell me how to do that!

[Warthaug]

There is a free program called bioedit, for windows, that does a good job of alinging sequences. Download it, install, import your sequences, then:

1) Convert your reverse sequence to the "reverse-complement"
2) Align the two sequences, using the "allow ends to slide" alignment. This will align the two sequences such that you can read the gene end-to-end.

You should now be able to copy the whole gene sequence for later use

Bryan

[dym27]

Bryan thanks. I'll try it later when I get home - Now let me ask another question please..

What about the fwd sequence? I mean as you can see I'm an amateur molecular biologist so let me tell you the whole story..

I'm aiming to do the barcode for the st I mentioned so I got both fwd and reverse sequences for several individuals wich the sequencing company sent to me...do you know what should I do now? I mean a teacher told me to use each sequence (F and R) and join them so I get the gene for each individual...I don't understand it clearly

Thanks for your reply


Daniel