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Can anyone help me to correct my primer design?

Can anyone help me to correct my primer design? - Bioinformatics

Can anyone help me to correct my primer design? - Have questions about bioinformatic tools or databases? Post questions here. Discuss and post interesting bioinformatics information.


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Old 12-12-2009, 12:21 PM
Pipette Filler
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Default Can anyone help me to correct my primer design?



Hello everyone, I am just starting learning to design primer. But I still have problem with reverse primer design.
If I have sequence like this
5 GATAGTGGTTGCGTTGTGAGCTGGAAAAAAAAGAACTGAAATGTGGCAGTTGGTCAACTCCTTGGTCACAGCT 3
If I use start codon ATG and ACTAGT as restriction enzyme, my forward primer should be 5 ACTAGT ATG GAT AGT GGT TGC GTT GTG 3
If I use TAA as stop codon and CGGCCG as restriction enzyme, my reverse primer should be 5 GCCGGC AAT AGC TGT GAC CAA GGA GTT 3
Please help and I will be happy to have your suggestion. Thank you.
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