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# Unit conversion

## Unit conversion - Biochemistry Forum

### Unit conversion - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!

#1
05-23-2013, 06:35 AM
Pipette Filler

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Unit conversion

Can someone help me how to convert ng to uM??

10 ng to ?uM

Thanks
#2
05-23-2013, 02:09 PM
M.D/Ph.D

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Re: Unit conversion

Grams (like in ng) are an absolute measure, at least in earth at 1 atm and 1 grav; while molarity (like in uM) is relative: talking about mass a gram of NaCl is equal to a gram of EDTA, but a 1 M NaCl is not equal to 1 M EDTA.

Molarity is a measure of concentration that depends on molecular weight (MW) of the substance, and the final volume of the solution your preparing. Let's take NaCl as example: its MW=58.44 , dissolve 58.44 g in 1 L of ddH2O and you will have a 1 M NaCl solution. Further dilutions can be made from there (in paper, you don't have to prepare that much solution if not needed): 1 M = 58.44 g in 1 L or 5.844 g in 100 ml or 0.5844 g in 10 ml, etc.

What you need to do is to investigate the MW of the reagent (or whatever it is) and use some math. This formula can give you hint:

C1V1 = C2V2

If you want further help or to directly solve your question at least mention the reagent you are working with and the volume you need.

Last edited by luisillo; 05-23-2013 at 02:11 PM.
#3
05-23-2013, 02:11 PM
Pipette Filler

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Re: Unit conversion

Okay...

I need to know how much 10 ng of creatinine (MW 113.11) is in micro molar
#4
05-23-2013, 02:18 PM
Pipette Filler

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Re: Unit conversion

Or....

I have 10 ul of 10 uM creatinine... How many ng creatinine is that??
#5
05-23-2013, 02:22 PM
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Re: Unit conversion

It depends on the volume:

10 ng in 1 ul = 88.41 uM
10 ng in 100 ul = 0.8841 uM
10 ng in 1 ml = 0.08841 uM

See
#6
05-23-2013, 02:24 PM
Pipette Filler

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Re: Unit conversion

Well... I have 10 ul of a creatinine solution of 10uM.... How many ng creatinine is there in the 10 ul??
#7
05-23-2013, 02:34 PM
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Re: Unit conversion

Sorry I didn't read well as you can see. I beleive the answer is 11.311 ng

11.311 ng in 10 ul = 10 uM

xD
#8
05-23-2013, 02:35 PM
Pipette Filler

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Re: Unit conversion

thanks

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