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Animal-Derived Laboratory Reagents

Animal-Derived Laboratory Reagents - Biochemistry Forum

Animal-Derived Laboratory Reagents - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 12-06-2012, 06:21 PM
Pipette Filler
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Default Animal-Derived Laboratory Reagents



I have a question about animal-derived laboratory reagents and products. Iím working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These include:

FBS (fetal bovine serum) & BSA (bovine serum albumen): I figured these products are likely a byproduct of the slaughterhouse industry and therefore the animals are being slaughtered more for the meat than to get the blood to make these products. Am I right in thinking this?

Primary & secondary antibodies (raised in mouse, rabbit, goat, etc.): From what I understand there are three main types with differing degrees of cost to animal life
ē In vitro Monoclonal antibodies: Once the hybridoma is created they can be grown indefinitely in vitro so there is minimal cost to animal life. Am I right in thinking this?
ē In vivo (ascites) monoclonal antibodies: Does an animal have to die every time I place a new order for an antibody?
ē Polyclonal antibodies: Again, does an animal have to die every time I place a new order for an antibody?

Sheepís blood agar: Unlike the bovine products, I wouldnít think sheep are being killed for the meat so maybe they are being primarily slaughtered to generate these lab products. Am I right for thinking this?

Iím asking these questions because I enjoy working in the lab but just want to know the cost associated with the products I use in regards to animal life. Also, can you think of any other animal-derived reagants/products that I left out that could be used in in-vitro experiments?

Thanks for any help you can provide.
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