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Glycine Buffer

Glycine Buffer - Biochemistry Forum

Glycine Buffer - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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  #1  
Old 10-28-2012, 12:18 PM
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Default Glycine Buffer



When doing our glycine lab, the carboxyl pka changed more than 2 log units from our initial pka of acetic acid, and our opium remained relatively the same. Can anyone help me explain why that happened? Any help would be much appreciated!

Below is the assignment:


You can paste this data table into your report. For your discussion explain the data you can use ~1/2-3/4 of*a page.* To help you get started: Why did the carboxyl pKa change so much below while the onium pKa changed only slightly?* What is the significance of the changes?
*
*
**************************************** Theoretical************************ Experimental
Acetic Acid:************************ 4.75*************************************** 4.56 +/- 0.144 *** (N=15)
Gly-Carboxyl********************* 2.34*************************************** 2.36 +/-0.35******* (N=4)
Ammonium*********************** 9.25*************************************** 9.50 +/- 0.23****** (N=6)
Gly-Onium************************* 9.60*************************************** 9.57 +/- 0.061**** (n=4)
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  #2  
Old 12-03-2012, 06:35 PM
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Default Re: Glycine Buffer

Because the shape changed dramatically but the opium levels changed little?
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Old 02-11-2013, 06:31 AM
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Old 02-11-2013, 07:23 AM
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