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Buffer for cdk1 cyclinB purification

Buffer for cdk1 cyclinB purification - Biochemistry Forum

Buffer for cdk1 cyclinB purification - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 10-25-2012, 09:27 PM
Pipette Filler
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Default Buffer for cdk1 cyclinB purification



Hi,
I've produced His-cyclinB/CDK-1 in insect cells with appropriate baculoviruses. Now I've to purify the complex. Which buffer would you use for the purification? In the data sheet of commercially available kinase, there is no the purification buffer composition... I think use firstly phosphate buffer with 250 mM Nacl (+imidazole) and then GF or dialysis with the same buffer plus 10% glycerol... What do you think ? Globally, which buffer would you use for kinase purification ?Thank in advance for your help. Best! N
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