Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum
Register Search Today's Posts Mark Forums Read

Biochemistry Forum Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


ATP hydrolysis & ΔG

ATP hydrolysis & ΔG - Biochemistry Forum

ATP hydrolysis & ΔG - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 09-05-2012, 04:07 PM
Pipette Filler
Points: 53, Level: 1 Points: 53, Level: 1 Points: 53, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Sep 2012
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default ATP hydrolysis & ΔG



Experimentally, the standard free energy change associated with the hydrolysis of ATP can be determined by measuring the equilibrium constants for the following two reactions:
i. ATP + glucose --> ADP + glucose 6-phosphate Keq = 890
ii. glucose + inorganic phosphate --> G6P + H2O Keq = 0.0037

1) Based on these data, what is the ΔG for the hydrolysis of ATP?
Draw a reaction coordinate that demonstrates your solution.

Should I just find the ΔG of the 2 rxns and add them? Then I get 705.44 cal/mol.
or
should I invert the second rxn and cancel out the glucose and G6P? Then I get the ΔG = 7336.6 cal/mol.

2) Explain why this indirect method is experimentally more feasible than a direct measurement of the equilibrium constant for the hydrolysis of ATP.

If I calculate ΔG the first way, the ΔG is much less than the ΔG for reaction (i), which is the direct hydrolysis of ATP. So is it because the indirect method would lower the free energy needed to hydrolyze ATP?

PLEASE HELP!!
Reply With Quote
Reply

Tags
Δg , atp , hydrolysis


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Graphs !! Carbs and enzymatic hydrolysis jack-1-richards Biochemistry Forum 0 03-26-2010 12:50 PM
Gelatin hydrolysis vs starch hydrolysis sharmine Biology Forum 3 08-31-2009 11:38 PM
Enzymatic hydrolysis of cellulose- How do you do it with off the shelf stuff ? steve.mew@gmail.com Botany Forum 1 10-19-2005 02:36 PM
N-methylpyrrolidinone hydrolysis Amen Selah Organic Chemistry Forum 0 04-11-2005 07:41 AM
acetal hydrolysis Jean Organic Chemistry Forum 0 09-06-2004 02:04 PM


All times are GMT. The time now is 04:44 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11141 seconds with 16 queries