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# ATP hydrolysis & ΔG’°

## ATP hydrolysis & ΔG’° - Biochemistry Forum

### ATP hydrolysis & ΔG’° - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!

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09-05-2012, 04:07 PM
Pipette Filler

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ATP hydrolysis & ΔG’°

Experimentally, the standard free energy change associated with the hydrolysis of ATP can be determined by measuring the equilibrium constants for the following two reactions:
i. ATP + glucose --> ADP + glucose 6-phosphate K’eq = 890
ii. glucose + inorganic phosphate --> G6P + H2O K’eq = 0.0037

1) Based on these data, what is the ΔG’° for the hydrolysis of ATP?
Draw a reaction coordinate that demonstrates your solution.

Should I just find the ΔG’° of the 2 rxns and add them? Then I get 705.44 cal/mol.
or
should I invert the second rxn and cancel out the glucose and G6P? Then I get the ΔG’° = 7336.6 cal/mol.

2) Explain why this indirect method is experimentally more feasible than a direct measurement of the equilibrium constant for the hydrolysis of ATP.

If I calculate ΔG’° the first way, the ΔG’° is much less than the ΔG’° for reaction (i), which is the direct hydrolysis of ATP. So is it because the indirect method would lower the free energy needed to hydrolyze ATP?

 Tags Δg’° , atp , hydrolysis

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