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Enzyme technology ( catechin assay)

Enzyme technology ( catechin assay) - Biochemistry Forum

Enzyme technology ( catechin assay) - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 03-18-2012, 12:52 PM
Pipette Filler
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Red face Enzyme technology ( catechin assay)



Well, I'm using catechin for my polyphenol oxidase assay to determine the substrate specificity. But the problem is when I try to use 50mM above, the concentration seems to be very high which the spectrophotometer shows more than 4.500. What should I do?? I couldn find any info from the website or journals. Should I dilute it? And I should dilute the substrate only or together with the enzyme?
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Old 04-04-2012, 03:46 AM
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assay , catechin , catechin hydrate , dilution , enzyme , spectrophotometer , technology


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