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-   -   Enzyme technology ( catechin assay) (http://www.molecularstation.com/forum/biochemistry-forum/84951-enzyme-technology-catechin-assay.html)

angellee0113 03-18-2012 12:52 PM

Enzyme technology ( catechin assay)
 
Well, I'm using catechin for my polyphenol oxidase assay to determine the substrate specificity. But the problem is when I try to use 50mM above, the concentration seems to be very high which the spectrophotometer shows more than 4.500. What should I do?? I couldn find any info from the website or journals. Should I dilute it? And I should dilute the substrate only or together with the enzyme?

ewlxdsmt 04-04-2012 03:46 AM

Re: Enzyme technology ( catechin assay)
 
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