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Ion exchange chromatography - Cation exchangers and TRIS Buffer? Compatible?

Ion exchange chromatography - Cation exchangers and TRIS Buffer? Compatible? - Biochemistry Forum

Ion exchange chromatography - Cation exchangers and TRIS Buffer? Compatible? - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 03-11-2012, 12:35 PM
Pipette Filler
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Default Ion exchange chromatography - Cation exchangers and TRIS Buffer? Compatible?



Hi People,

thats more or less my problem,

I'm having some problems to purify my peptide by reversed phase chromatography. I have decided to move to Ion exchange chromatography since my peptide is quite basic (it has Lysine, Arginine). Actually the sequence is like KARKSAGA where in the K in the middle of the sequence it has been modfied. The pI of my peptide is around 10-11. So I have decided to use cation exchanger. The problem is that my peptide is dissolved with Tris buffer and I would like to use also as eluent Tris buffer. So far I read that Tris buffer is not recommended for Cation exchangers...

My question is Why?

Thanks
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buffer , cation , chromatography , compatible , exchange , exchangers , ion , tris


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