thats more or less my problem,
I'm having some problems to purify my peptide by reversed phase chromatography. I have decided to move to Ion exchange chromatography since my peptide is quite basic (it has Lysine, Arginine). Actually the sequence is like KARKSAGA where in the K in the middle of the sequence it has been modfied. The pI of my peptide is around 10-11. So I have decided to use cation exchanger. The problem is that my peptide is dissolved with Tris buffer and I would like to use also as eluent Tris buffer. So far I read that Tris buffer is not recommended for Cation exchangers...
My question is Why?