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#1
03-11-2012, 12:35 PM
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 Ion exchange chromatography - Cation exchangers and TRIS Buffer? Compatible? Hi People, thats more or less my problem, I'm having some problems to purify my peptide by reversed phase chromatography. I have decided to move to Ion exchange chromatography since my peptide is quite basic (it has Lysine, Arginine). Actually the sequence is like KARKSAGA where in the K in the middle of the sequence it has been modfied. The pI of my peptide is around 10-11. So I have decided to use cation exchanger. The problem is that my peptide is dissolved with Tris buffer and I would like to use also as eluent Tris buffer. So far I read that Tris buffer is not recommended for Cation exchangers... My question is Why? Thanks  |
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| buffer , cation , chromatography , compatible , exchange , exchangers , ion , tris |
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