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-   -   Ion exchange chromatography - Cation exchangers and TRIS Buffer? Compatible? (http://www.molecularstation.com/forum/biochemistry-forum/84887-ion-exchange-chromatography-cation-exchangers-tris-buffer-compatible.html)

Valine 03-11-2012 12:35 PM

Ion exchange chromatography - Cation exchangers and TRIS Buffer? Compatible?
 
Hi People,

thats more or less my problem,

I'm having some problems to purify my peptide by reversed phase chromatography. I have decided to move to Ion exchange chromatography since my peptide is quite basic (it has Lysine, Arginine). Actually the sequence is like KARKSAGA where in the K in the middle of the sequence it has been modfied. The pI of my peptide is around 10-11. So I have decided to use cation exchanger. The problem is that my peptide is dissolved with Tris buffer and I would like to use also as eluent Tris buffer. So far I read that Tris buffer is not recommended for Cation exchangers...

My question is Why?

Thanks


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