Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum
Register Search Today's Posts Mark Forums Read

Biochemistry Forum Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


BioChem: Solving a mutation question

BioChem: Solving a mutation question - Biochemistry Forum

BioChem: Solving a mutation question - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-01-2012, 04:27 AM
Pipette Filler
Points: 38, Level: 1 Points: 38, Level: 1 Points: 38, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2012
Posts: 3
Thanks: 0
Thanked 0 Times in 0 Posts
Default BioChem: Solving a mutation question



Here is a exam prep question I am totally stuck on.
Anybody want to lend a hand getting me started.
I am only having trouble with the E part of the question.
(10 marks). You are studying the transcription of the FBD1 gene. You have a clone of the wild type FBD1 gene, and three mutant genes. The location of each mutation is shown in the table. You transfect each version of the gene into cultured heart, lung or kidney cells in separate experiments and measure the total number of FDB1 mRNAs coming from transcription of the introduced gene. From the data in the table, answer the following questions:
(a) Compare the expression of the wild type gene in heart, lung and kidney. What conclusions can you draw about the tissue-specificity of FDB1 gene expression?
(b) What role does the region A sequence play in transcription of the FDB1 wild type gene in the three tissues? Explain your reasoning.
(c) What role does the region B sequence play in transcription of the FDB1 wild type gene in the three tissues? Explain your reasoning.
Number of mRNAs from specified gene
FBD1 Gene
Mutated region
Heart
Lung
Kidney
wild type
none
300
15
3000
mutant A
-300 to -350
300
300
3000
mutant B
-20 to -50
0
0
0
mutant C
+566 to +683
300
15
300
(d) What role does the region C sequence play in transcription of the FDB1 wild type gene in the three tissues? Explain your reasoning.
(e) What differences might you expect there to be in the nuclear components of heart, lung and kidney cells that explain how regions A, B and C function? Suggest one experiment you could do to test your hypothesis.

Thanks.
Reply With Quote
Reply

Tags
biochem , mutation , question , solving


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Genetic mutation question Northern General Science Questions and Layperson Board 5 09-10-2010 07:04 PM
Mutation to Increase Plasmid Copy Number Question rbb147 DNA Forum 0 06-27-2010 06:06 PM
intro biochem question chem UofM Protein Science 0 03-04-2010 09:56 PM
Amino Acid Mutation Question rbb147 Protein Science 0 01-07-2010 06:40 AM
GL Biochem acquired Commonwealth Biotechnologies glbiochem Peptide Forum 0 06-15-2009 06:05 AM


All times are GMT. The time now is 12:27 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11524 seconds with 16 queries