Here is a exam prep question I am totally stuck on.
Anybody want to lend a hand getting me started.
I am only having trouble with the E part of the question.
(10 marks). You are studying the transcription of the FBD1 gene. You have a clone of the wild type FBD1 gene, and three mutant genes. The location of each mutation is shown in the table. You transfect each version of the gene into cultured heart, lung or kidney cells in separate experiments and measure the total number of FDB1 mRNAs coming from transcription of the introduced gene. From the data in the table, answer the following questions:
(a) Compare the expression of the wild type gene in heart, lung and kidney. What conclusions can you draw about the tissue-specificity of FDB1 gene expression?
(b) What role does the region A sequence play in transcription of the FDB1 wild type gene in the three tissues? Explain your reasoning.
(c) What role does the region B sequence play in transcription of the FDB1 wild type gene in the three tissues? Explain your reasoning.
Number of mRNAs from specified gene
-300 to -350
-20 to -50
+566 to +683
(d) What role does the region C sequence play in transcription of the FDB1 wild type gene in the three tissues? Explain your reasoning.
(e) What differences might you expect there to be in the nuclear components of heart, lung and kidney cells that explain how regions A, B and C function? Suggest one experiment you could do to test your hypothesis.