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Biochemistry Forum Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


Cyropreservation

Cyropreservation - Biochemistry Forum

Cyropreservation - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 09-19-2011, 03:37 PM
Pipette Filler
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Default Cyropreservation



I've isolated buffy coat and plasma from whole blood using fractionation. I'm considering cryo preservation solutions to store at -80. I've done some digging and found suggestions of using anywhere from .1 to 20% DMSO. Suggestions on what has been done previously and what to dilute the stock DMSO with as well, i've considered pbs, TE, DI water, nuclease free water.
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-80 , buffy coat , cryopreservation , cyropreservation , dmso


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