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Problem with BCA protein quantification

Problem with BCA protein quantification - Biochemistry Forum

Problem with BCA protein quantification - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 09-02-2011, 12:47 PM
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Default Problem with BCA protein quantification



Hi everyone,
I have a very urgent problem with the Pierce BCA kit. My diluted standards show extremely high ODs (all the same) and it's impossible to generate a standard curve. I use the normal Laemmli buffer (without bromphenolblue) and theoretically no substance should interfere...
Any ideas? Thx!
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Old 09-22-2011, 07:28 PM
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Default Re: Problem with BCA protein quantification

If your samples are in Laemmli buffer, you can use anything to dilute.
Your standards do not need to be in Laemmli.

You can use a dilution buffer to make the samples match the standards.
I use 1:20 dilution factor in either PBS or H20. Since laemmli is pretty strong buffer I'd use H2O if I were you.
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