I have to cultivate Sf9 cells to produce recombiant proteins after baculoviruses infection.
My question concerns culture conditions of this type of cell. Please, can you indicate me how do you cultivate them… I have no experience regarding these cells. Actually, I try to cultivate them into the Lonza Insect-XPRESS™ Protein-free Insect Cell Medium with L-glutamine, using TPP plastic 75 cm² flasks with closable lids. Cells are low passage (P6) and come from a lab in Pasteur Institut. In fact, I’m cultivating these cells exactly as in the lab in Pasteur. And cells don’t grow : when I split them (1/5), new batch do not grow…
I’m sure that I’m in sterile conditions as no trouble can be observed in flasks.
What can you advise to me ?
Thank you very much in advance, best regards.