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Phytochemical purification: removal of residual glucose
lets say i have a plant pulp juice, and i want to administer these extracts onto a cell line, or rat tissue or whatnot.
to extract general phytochemicals prior to rotary evaporation, i employed solvent extraction using n-butanol. theoretically speaking, the glucose should dissolve in the water fraction, and the phytochemicals which are generally less polar dissolve in the n-BuOH fraction. i performed a solubility test using an anhydrous glucose standard, and it is observably insoluble. subsequently, i employed the solvent extraction and performed a molisch test (test for carbs) on each fraction after 3-5 mins of swirling. the purple portion of sugars present significanly decreased, but of course i dont exactly know if this would hold true for a pulp juice, with a lot of other components in the system.
is a simple n-butanol extraction effective for removing much if not all of the residual sugar?
can anybody suggest a relatively cheap way of removing the sugars without damaging the phytochemicals?
many many thanks.
|glucose , phytochemical , purification , removal , residual|
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