Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum
Register Search Today's Posts Mark Forums Read

Biochemistry Forum Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


Enzyme Kinetics

Enzyme Kinetics - Biochemistry Forum

Enzyme Kinetics - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 02-11-2010, 04:09 PM
Pipette Filler
Points: 24, Level: 1 Points: 24, Level: 1 Points: 24, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Feb 2010
Posts: 1
Thanks: 0
Thanked 0 Times in 0 Posts
Default Enzyme Kinetics



Hello, Im taking an undergraduate course in biochem, and I came across a question I dont understand.

For an enzyme catalyzed reaction, the initial velocity was determined at two different concentrations of the substrate. Estimate the value of Km. The hint says we dont have to do a double reciprocal plot for this question, but can use the Michaelis-Menten equation.

[S](mM) V(mM/min)
1.0 2.0
4.0 2.8


Ive tried doing this several times and I cant get a reasonable answer. Could someone please walk me through this?

Thank you!
Reply With Quote
Reply

Tags
enzyme , kinetics


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Enzyme Assays peterish Protein Forum 5 05-19-2011 10:56 AM
How can fractional saturation>1 (enzyme kinetics)? denisov Biochemistry Forum 0 11-19-2009 10:33 AM
spectrophotometry enzyme substrate dilution problem stinkytofu Biochemistry Forum 3 02-23-2009 12:26 AM
Fluorescence And Enzyme & Binding Assays Training Courses aftabac Conferences , Symposiums and Meetings 0 12-10-2007 05:38 PM
Enzyme kinetics software Christian Gómez Protein Forum 0 03-22-2006 11:15 PM


All times are GMT. The time now is 02:57 AM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11040 seconds with 16 queries