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Small unilamellar vesicles + Dehydrogenase constructs

Small unilamellar vesicles + Dehydrogenase constructs - Biochemistry Forum

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Old 09-27-2009, 08:52 PM
Pipette Filler
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Default Small unilamellar vesicles + Dehydrogenase constructs



Hi guys

I'm really stuck right now and I hope someone can help me. I just began my master thesis and I have to prepare an experiment. We would like to produce small unilamellar vesicles (SUVs) and insert a dehydrogenase (retinol dehydrogenase, RDH5) into them, since its a membrane protein. Could anyone tell me, how this could be done? I know how to prepare SUVs (read some papers about this procedure) but I don't really know, how to insert the dehydrogenase constructs ?! Perhaps express them in E.coli first and then mix them somehow with the SUVs?

I hope someone can help me

Cheers,

dafenris
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