Go Back   Science Forums Biology Forum Molecular Biology Forum Physics Chemistry Forum > General Science Forums > Biochemistry Forum
Register Search Today's Posts Mark Forums Read

Biochemistry Forum Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


Quantification of Ubiquitination

Quantification of Ubiquitination - Biochemistry Forum

Quantification of Ubiquitination - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


Reply
 
LinkBack Thread Tools Display Modes
  #1  
Old 07-05-2009, 11:45 PM
Pipette Filler
Points: 52, Level: 1 Points: 52, Level: 1 Points: 52, Level: 1
Activity: 0% Activity: 0% Activity: 0%
 
Join Date: Jul 2009
Posts: 4
Thanks: 0
Thanked 0 Times in 0 Posts
Default Quantification of Ubiquitination



Hi,

I would like to know if there is a method quantify/assess the degree of ubiquitination of a protein of interest in tissue lysate?
My idea was to do a co-immunoprecipitation of polyubiquitin and my protein but I am afraid this is not quantitative enough.
Or is there a method to quantify the proteasomal degradation of a protein x in vitro?

I have no experience with this area and would greatly appreciate your help!
Reply With Quote
Reply

Tags
quantification , ubiquitination


Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On

Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
Quantification of Ubiquitination Thitus Immunoprecipitation Forum 2 07-09-2009 01:41 AM
qPCR NEWS April 2009 - focus on microRNA normalisation Editor www.Gene-Quantification.info Protocols and Methods Forum 0 04-29-2009 02:18 PM
Help! A question about quantification by MALDI-TOD-MS tarot Mass Spectrometry Forum 2 02-21-2008 02:40 AM
protein quantification Jayakumar, R Protocols and Methods Forum 2 12-31-2003 06:07 AM
protein quantification Jayakumar, R Protocols and Methods Forum 2 12-18-2003 10:22 PM


All times are GMT. The time now is 06:16 PM.


Powered by vBulletin® Version 3.8.4
Copyright ©2000 - 2014, Jelsoft Enterprises Ltd.
Copyright 2005 - 2012 Molecular Station | All Rights Reserved
Page generated in 0.11009 seconds with 16 queries