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Specificity difference between Affinty Labels and Group specific reagents?

Specificity difference between Affinty Labels and Group specific reagents? - Biochemistry Forum

Specificity difference between Affinty Labels and Group specific reagents? - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 02-05-2009, 09:40 PM
Pipette Filler
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Post Specificity difference between Affinty Labels and Group specific reagents?



Hi guys i was wondering if you could give me some help in defining the specificity difference between affinity labels or group specific reagents.

I know that affinity labels are more specific but im not sure why this is the case? do group specific reagents bind to any residues on an enzyme and affinity lebels on bind to active site residues? any help would be appreciated!

Also can anyone think of an example of relating this mapping technique to receptors?
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Old 02-05-2009, 10:33 PM
tgd tgd is offline
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Default Re: Specificity difference between Affinty Labels and Group specific reagents?

Hello,

I think the distinction is that group-specific ligands bind a general class of proteins or enzymes, whereas affinity ligands tend to be specific for a single protein or enzyme.

NADP-Agarose, for example, would be expected to bind many if not all of the NADP-linked dehydrogenases in a tissue extract and unless you can find specific elution conditions, there may be many such dehydrogenases present in the post NADP-agarose sample. (Thus NADP immobilized on agarose may be described as a group-specific ligand).

On the other hand, Alpha-Cyano-4-hydroxycinnamate, for example, appears to be a very specific affinity ligand in the purification of an aldehyde dehydrogenase, and apparently binds very little else!

See R. C. Poole and A. P Halestrap Biochem J. 1989 April 1; 259(1): 105110.


Purification of aldehyde dehydrogenase from rat liver mitochondria by alpha-cyanocinnamate affinity chromatography.

I think that both group-specific and affinity ligands may bind the target protein very tightly.

Good luck,

tgd.
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Old 02-06-2009, 11:36 AM
Pipette Filler
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Default Re: Specificity difference between Affinty Labels and Group specific reagents?

Ahhh thank you tgd! You are right i don't know how i never made that distinction before! I even knew that organophophates bind to the serine proteases family as well but still it never crossed my mind

thanks again much appreciated
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affinity , affinty , biochemistry , difference , group , labels , reagents , specific , specificity


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