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question RE: biotin-streptavidin interaction

question RE: biotin-streptavidin interaction - Biochemistry Forum

question RE: biotin-streptavidin interaction - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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  #1  
Old 12-03-2008, 05:55 PM
Pipette Filler
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Default question RE: biotin-streptavidin interaction



A little complicated:

I am hooking a 5aa-peptide to biotin for ease of purification, and I need to know how "deep" in the strepavidin binding pocket the biotin moiety rests, i.e. will my pentapeptide be occluded in the binding pocket with the biotin, or will it be exposed? Basically, I am trying to decide how long of a "linker" to add between my pentapeptide of interest and the biotin tag to ensure that the peptide is still accessible outside the streptavidin tetramer.

Thanks!
Toby
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Old 12-04-2008, 12:52 AM
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Default Re: question RE: biotin-streptavidin interaction

this link provide the graphical view of the binding in different energy level
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Old 12-04-2008, 04:56 PM
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Default Re: question RE: biotin-streptavidin interaction

thanks so much!
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