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Buffer Tris Question!!! please help!

Buffer Tris Question!!! please help! - Biochemistry Forum

Buffer Tris Question!!! please help! - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 09-17-2008, 11:56 PM
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Question Buffer Tris Question!!! please help!



Tris is a widely used buffer in biochemistry and molecular biology research. Its molecular weight is 121.14g/mol. how many gram of Tris you need to male 1L of 1 M stock solution ? How many mL of water you need to make a 0.1 M Tris solution from 10 mL of 1M stock solution? If the 1 M Tris stock solution is labeled as 1000 X , what is the working concentration ? How many mL of stock solution you need to make 1 L of working solution?

Please help me with this!!!!!
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Old 09-18-2008, 06:07 AM
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Default Re: Buffer Tris Question!!! please help!

Hi,
I hope this are not ur homework questions , anyway

1.U will require 121.14g/1000ml water for 1M.
2. 90ml water
3.1mM
4.1ml

I think these are pretty easy to work out, u shud learn better.
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Old 09-18-2008, 01:48 PM
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Question Re: Buffer Tris Question!!! please help!

No these R not my homework questions....but could you please tell me how U calculate this?
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