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-   -   Buffer Tris Question!!! please help! (http://www.molecularstation.com/forum/biochemistry-forum/3960-buffer-tris-question-please-help.html)

olenka 09-17-2008 11:56 PM

Buffer Tris Question!!! please help!
 
Tris is a widely used buffer in biochemistry and molecular biology research. Its molecular weight is 121.14g/mol. how many gram of Tris you need to male 1L of 1 M stock solution ? How many mL of water you need to make a 0.1 M Tris solution from 10 mL of 1M stock solution? If the 1 M Tris stock solution is labeled as 1000 X , what is the working concentration ? How many mL of stock solution you need to make 1 L of working solution?

Please help me with this!!!!!

tuttu 09-18-2008 06:07 AM

Re: Buffer Tris Question!!! please help!
 
Hi,
I hope this are not ur homework questions , anyway

1.U will require 121.14g/1000ml water for 1M.
2. 90ml water
3.1mM
4.1ml

I think these are pretty easy to work out, u shud learn better.

olenka 09-18-2008 01:48 PM

Re: Buffer Tris Question!!! please help!
 
No these R not my homework questions....but could you please tell me how U calculate this?


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