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Recovery of activity??

Recovery of activity?? - Biochemistry Forum

Recovery of activity?? - Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


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Old 11-15-2007, 07:17 PM
Pipette Filler
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Default Recovery of activity??



my assignment deals with affinity chromatography for lactate dehydrogenase from rat liver and then using procion red for an assay. I have been asked to discuss 'recovery of activity' for which I need an explanation.Also,how is it possible to recover a greater amount of activity than was applied as well as possible further investigation for any expalnation.PLEASE help!
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Old 11-15-2007, 08:59 PM
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Default Re: Recovery of activity??

think about protein concentrations
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Old 04-24-2008, 07:25 AM
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Default Re: Recovery of activity??

If you speak about SPECIFIC activity, this is kind of obvious: the purity of the protein is higher, so the specific activity increased.

If you mean that the TOTAL activity increased, it is a different thing, and it is hard to explain.
Probably, an inhibitory factor was removed during purification?
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