calculate volume of buffer in reaction mixture

[spicooli]

Hi guys,

I have this urgent problem.
How do you calculate the volume of potassium phosphate buffer to add in a 1ml reaction mixture? The reaction mixture must contain 100mM potassium phosphate buffer, 0.3mM H202, 0.5mM ascorbate and the enzyme extract.

I have 1L of 100mM potassium phosphate buffer. I want to know how much to add into the reaction mixture.

Hope you guys can help me. Thanks a lot!

[kmunson779]

If your final concentration must be 100 mM, and your stock is also 100mM, then you've got a problem. You need a more concentrated stock solution.

Sorry, I forgot to mention that once you get your more concentrated stock, you calculate how much you need using

V1*C1 = V2*C2

Where V is the volume, C is the concentration, and 1&2 are your two conditions (stock vs. working solution). Make sure your units are all the same.

[littlesti]

try the 'wiley' biochem website. look up on google.