Go Back   Molecular Biology Forum > General Science Forums > Biochemistry Forum
Register Blogs FAQ Members List Calendar Science Groups New! Arcade Search Today's Posts Mark Forums Read

Biochemistry Forum Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!


differential/isopycnic/rate zonal centrifugation

Biochemistry Forum

Discuss and post questions regarding the study of Biochemistry. If you need homework help this is the place to ask!



Register Molecular Biology Forums
Reply
 
LinkBack Thread Tools Display Modes
  #1 (permalink)  
Old 06-15-2007, 02:09 AM
Pipette Filler
Points: 643, Level: 12Points: 643, Level: 12Points: 643, Level: 12
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Jun 2007
Posts: 4
jones106 RSS Feed
Default differential/isopycnic/rate zonal centrifugation

Hey guys, i'm a little confused about these types of centrifugation. Here is what I know (or think I know) about them:
Differential centrifugation is a good way to roughly separate cellular components based on their sedimentation coefficient (which is based on mass and shape). Larger and more massive components will sediment at lower speeds, while smaller components require higher centrifugal force.
Isopycnic uses a gradient of CsCl to separate based on buoyant densities. More dense components will equilibrate in the more dense regions of the CsCl while the less dense components will equilibrate in the less dense regions of the CsCl.
Rate zonal separates components based on their S-value, which determines how quickly the particles will move through a sucrose gradient. The greater the S-value, the more quickly it will move through the medium.

First of all, do I basically have the right idea about these methods?
Secondly, the book we are using in Biochem. doesn't say much about these methods, so I turned to my old Cell Bio. book. This book seems to imply that Isopycnic and Rate Zonal are only used for separating nucleic acids. Can these methods be used for separating other things, such as proteins from other proteins?

Sorry for the long-windedness, but I just feel like I am missing something important here.

Thanks in advance,
Taylor
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Alt Today
Advertising
Google Adsense
 
This advertising will not be shown
in this way to registered members.
Register your free account today
and become a member on
Molecular Biology Forum
Standard Sponsored Links

  #2 (permalink)  
Old 06-15-2007, 05:35 AM
admin's Avatar
Administrator
Points: 9,714, Level: 68Points: 9,714, Level: 68Points: 9,714, Level: 68
Activity: 100%Activity: 100%Activity: 100%
 
Join Date: Nov 2005
Posts: 994
Blog Entries: 3
admin RSS Feed
Default Re: differential/isopycnic/rate zonal centrifugation

Hello,
yes you are correct.

Sedimentation Equilibrium or Isopycnic Centrifugation. Isopycnic or sedimentation equilibrium centrifugation involves allowing the sedimenting species to move through the gradient until they reach a point where their density and that of the gradient are identical.

See: Lecture 9

Also take a look at this amazing handout:
Centrifugation Handout

Last edited by admin; 06-15-2007 at 05:39 AM.
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #3 (permalink)  
Old 06-15-2007, 03:31 PM
Pipette Filler
Points: 643, Level: 12Points: 643, Level: 12Points: 643, Level: 12
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Jun 2007
Posts: 4
jones106 RSS Feed
Default Re: differential/isopycnic/rate zonal centrifugation

Awesome! Thank you very much, that was really helpful!

Taylor
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #4 (permalink)  
Old 06-15-2007, 09:57 PM
admin's Avatar
Administrator
Points: 9,714, Level: 68Points: 9,714, Level: 68Points: 9,714, Level: 68
Activity: 100%Activity: 100%Activity: 100%
 
Join Date: Nov 2005
Posts: 994
Blog Entries: 3
admin RSS Feed
Default Re: differential/isopycnic/rate zonal centrifugation

Hey Taylor,
No problem I am learning too

If you have any more questions or further information to add, please do.

Cheers
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
  #5 (permalink)  
Old 12-12-2007, 08:37 PM
Pipette Filler
Points: 267, Level: 5Points: 267, Level: 5Points: 267, Level: 5
Activity: 0%Activity: 0%Activity: 0%
 

Join Date: Dec 2007
Posts: 5
baka RSS Feed
Default Re: differential/isopycnic/rate zonal centrifugation

Thank you so much .
Digg this Post!Add Post to del.icio.usBookmark Post in TechnoratiFurl this Post!Spurl this Post!Reddit!
Reply With Quote
Reply

Thread Tools
Display Modes

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off
Trackbacks are On
Pingbacks are On
Refbacks are On
Forum Jump

Similar Threads
Thread Thread Starter Forum Replies Last Post
differential centrifugation baka Protocols and Methods Forum 0 12-10-2007 11:12 AM
differential centrifugation baka Basic Lab Protocols and Techniques 0 12-07-2007 05:49 AM
density gradient and rate zonal centifugation renzhou9 Protocols and Methods Forum 0 10-05-2007 03:14 AM


All times are GMT. The time now is 08:56 PM.


Powered by vBulletin® Version 3.7.1
Copyright ©2000 - 2008, Jelsoft Enterprises Ltd.
Copyright 2005-2007 Molecular Station | All Rights Reserved