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DNA stuck in agarose wells and Dna Smears

DNA stuck in agarose wells and Dna Smears - Basic Lab Protocols and Techniques

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  #1  
Old 09-15-2011, 07:42 AM
McH McH is offline
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Angry DNA stuck in agarose wells and Dna Smears



I cant seem to get it right, I dont know how and why the dna is stuck in the gel wells and only smears of dna can be seen without the bands in a place they are supposed to be.
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Old 09-15-2011, 02:05 PM
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Default Re: DNA stuck in agarose wells and Dna Smears

Either There is no DNA or High Salt concentration in your sample.
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Old 10-17-2011, 06:06 PM
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Default Re: DNA stuck in agarose wells and Dna Smears

Quote:
Originally Posted by McH View Post
I cant seem to get it right, I dont know how and why the dna is stuck in the gel wells and only smears of dna can be seen without the bands in a place they are supposed to be.
Any chance you made the gel up using water instead of TAE?

I've done that once before and it makes your samples really smeary.
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Old 10-09-2012, 12:16 PM
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Default Re: DNA stuck in agarose wells and Dna Smears

the instead of tae is not working
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agarose , dna , smears , stuck , wells


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