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-   -   DNA stuck in agarose wells and Dna Smears (http://www.molecularstation.com/forum/basic-lab-protocols-techniques/83577-dna-stuck-agarose-wells-dna-smears.html)

McH 09-15-2011 07:42 AM

DNA stuck in agarose wells and Dna Smears
 
I cant seem to get it right, I dont know how and why the dna is stuck in the gel wells and only smears of dna can be seen without the bands in a place they are supposed to be.

obama 09-15-2011 02:05 PM

Re: DNA stuck in agarose wells and Dna Smears
 
Either There is no DNA or High Salt concentration in your sample.

mmorgan 10-17-2011 06:06 PM

Re: DNA stuck in agarose wells and Dna Smears
 
Quote:

Originally Posted by McH (Post 436198)
I cant seem to get it right, I dont know how and why the dna is stuck in the gel wells and only smears of dna can be seen without the bands in a place they are supposed to be.

Any chance you made the gel up using water instead of TAE?

I've done that once before and it makes your samples really smeary.

samparker 10-09-2012 12:16 PM

Re: DNA stuck in agarose wells and Dna Smears
 
the instead of tae is not working


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