Hi, I am new in lab, and I am unsure how to perform nested pcr, which I have to do for an experiment as my primers are too huge to perform regular pcr.
How is performing nested pcr different from regular pcr? I have 8 different primer pairs to test as it is in 8 different fragments (already ordered primers). Do I perform the pcr test separately 8 different times, and once this is done the dna needs be sequenced. Do I then send these 8 different pcr samples to the lab for sequencing? Please help guys! Thanks