DNaseI

[Bommel]

Hi,
I would like to digest DNA in an RNA preparation. I heard about bead bound DNaseI. Can anybody tell me something about this beads. Maybe anybody knows the company which offers them.
Thanks,
Bommel

[PAO05]

Hey Bommel!,

I have removed DNA contaminants from RNA isolations.

The bead bound DNAseI is probably more expensive than the regular DNA, plus it may be contaminated with RNases (is it RNase-free?)

We usually use RNAse-free DnaseI - very very cheap, and very effective...

it is easy to get rid of also - just heat your sample at 80C... thats it

Rnase free Dnase is from:

Qiagen
http://www1.qiagen.com/Products/Acce...DNaseSet.aspx?

and the one we use
Promega RQ1
http://www.promega.com/tbs/9pim610/9pim610.html

[PAO05]

As for the DNaseI-Sepharose beads, I found they are usually made.

"Cell lysates were incubated with DNaseI immobilized on Sepharose beads at 4°C for 1 h."

Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin

Qiang Wang2, Yi Xie1, Quan-Sheng Du1, Xiao-Jun Wu1, Xu Feng1, Lin Mei1,2,3, Jay M. McDonald1,4 and Wen-Cheng Xiong1

2003
The Journal of Cell Biology, Volume 160, Number 4, 565-575