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Bommel 05-17-2006 01:45 PM

DNaseI
 
Hi,
I would like to digest DNA in an RNA preparation. I heard about bead bound DNaseI. Can anybody tell me something about this beads. Maybe anybody knows the company which offers them.
Thanks,
Bommel

PAO05 05-18-2006 04:36 PM

Re: DNaseI
 
Hey Bommel!,

I have removed DNA contaminants from RNA isolations.

The bead bound DNAseI is probably more expensive than the regular DNA, plus it may be contaminated with RNases (is it RNase-free?)

We usually use RNAse-free DnaseI - very very cheap, and very effective...

it is easy to get rid of also - just heat your sample at 80C... thats it

Rnase free Dnase is from:

Qiagen
[Only registered and activated users can see links. Click Here To Register...]

and the one we use
Promega RQ1
[Only registered and activated users can see links. Click Here To Register...]

PAO05 05-18-2006 04:42 PM

Re: DNaseI
 
As for the DNaseI-Sepharose beads, I found they are usually made.

"Cell lysates were incubated with DNaseI immobilized on Sepharose beads at 4°C for 1 h."

Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin

Qiang Wang2, Yi Xie1, Quan-Sheng Du1, Xiao-Jun Wu1, Xu Feng1, Lin Mei1,2,3, Jay M. McDonald1,4 and Wen-Cheng Xiong1

2003
The Journal of Cell Biology, Volume 160, Number 4, 565-575


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