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Storability of precasting GelRed-agarose gels

Storability of precasting GelRed-agarose gels - Basic Lab Protocols and Techniques

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  #1  
Old 06-01-2010, 11:27 AM
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Default Storability of precasting GelRed-agarose gels



Hi all!

I have a qeustion regarding the storability of precasting-agarose gels stained with GelRed. In our lab gels (at the moment stained with EtBr) are sometimes stored for four days before being used. Has anybody information/experiences about the storage of GelRed gels? How ist the sability of these gels?

ThX a lot!

Paul

Last edited by ph2; 06-01-2010 at 12:12 PM.
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Old 06-01-2010, 04:25 PM
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Default Re: Storability of precasting GelRed-agarose gels

As far as I know, a gelred-agarose gel should stay good for at least a few weeks in the refrigerator if you wrap it in plastic wrap or put it in a ziplock bag with the air pressed out so it can't dry out.
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Old 06-08-2010, 11:40 AM
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Default Re: Storability of precasting GelRed-agarose gels

Hi all!

As it looks like GelRed is not stable in the frigde in precasting gels over a periode of 5 days. We will test it now for shorter periods and I will report about the results.

.-)
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Old 06-15-2011, 12:03 PM
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Default Re: Storability of precasting GelRed-agarose gels

Dear All,

Is there anybody who has tried to use GelRed putting directly into the wells after the sample instead of pre / post gel staining? Which way is more economical especially for a beginner without much time in a week to spend in the lab?
In case of doing post gel staining how long the GelRed staining solutions can be stored at room temperature? ( If I have time to go into the lab and try learning the methods once a week, then can I store staining solutions for weeks?)
Thank you in advance!
Krisztina
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Old 11-04-2011, 02:29 PM
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Default Re: Storability of precasting GelRed-agarose gels

nice post,, take it..
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Old 01-04-2012, 08:48 PM
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Default Re: Storability of precasting GelRed-agarose gels

I precast my gels with GelRed and keep them in the fridge for days to weeks. No problem, it's stable!
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Old 06-21-2012, 05:25 PM
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Default Re: Storability of precasting GelRed-agarose gels

I found that I could not re-use the gels with gelred as oppoesed to EtBr, where I could run the same gel maybe half a dozen times.
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