Originally Posted by hamid_v
i have touched agrose gel which contain ethidium bromide ( this is my first time )
That was around 0.1 gram ethidium bromide in 100ML TAE buffer
i poured the gel in Sink
, i drained
some water over gel , and then i moved the gel to dustbin using newspaper
( Newspaper got wet
because of that )
So i have touched
the gel when i moved from sink to dustbin ,
i had a little irritation
in a finger and i washed that fastly with out soap
So i want to know
am i going to have any problem because of this act ?
Am i going to take cancer
only for 1 time touch ?
You'll be fine. Probably the psychological stress of worrying has caused you more harm than the EtBr would.
EtBr is thought to act as a mutagen by intercalating DNA which means it has to get INSIDE of cells to do damage. The surface of your skin is covered by a layer of dead cells, so unless you soaked your hand in EtBr for a good while I think there is no problem.
You should be wearing gloves and goggles when working in the lab though, because there are alot of chemicals that are much worse for you than EtBr (i.e. acrylamide, methanol, HCl).
Relative to the DNA damage done by a day on the beach touching an EtBr gel is negligible.
So remember to wear your gloves and goggles and don't eat any of the lab chemicals and you'll be fine.