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#1
03-12-2009, 12:25 AM
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 What is the funcion of Glycine in immunostaining? Dear all, I got a protocol for immunostaining specific for 3D culture, and according to the protocol, I should use with PBS 1X-Glycine 50mM to wash after 4% paraformaldehyde fixation? I just have no idea what the function of Glycine here. Anyone can help me with that? Thanks. Lei  |
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#2
12-15-2009, 03:36 PM
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| Re: What is the funcion of Glycine in immunostaining? When you cross-link with your formaldehyde mixture, you do it for a specific amount of time. The glycine then acts as a quencher for the cross-linking, assuring that you do not over cross-link your sample, which could lead to non-specific cross-linking. Put simply, you stop the cross-linking reaction by quenching the reaction with glycine. |
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| funcion , glycine , immunostaining |
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