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What is the funcion of Glycine in immunostaining?

What is the funcion of Glycine in immunostaining? - Basic Lab Protocols and Techniques

What is the funcion of Glycine in immunostaining? -


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Old 03-12-2009, 12:25 AM
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Default What is the funcion of Glycine in immunostaining?



Dear all,

I got a protocol for immunostaining specific for 3D culture, and according to the protocol, I should use with PBS 1X-Glycine 50mM to wash after 4% paraformaldehyde fixation?

I just have no idea what the function of Glycine here.

Anyone can help me with that?

Thanks.

Lei
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Old 12-15-2009, 04:36 PM
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Default Re: What is the funcion of Glycine in immunostaining?

When you cross-link with your formaldehyde mixture, you do it for a specific amount of time. The glycine then acts as a quencher for the cross-linking, assuring that you do not over cross-link your sample, which could lead to non-specific cross-linking.

Put simply, you stop the cross-linking reaction by quenching the reaction with glycine.
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