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What is the funcion of Glycine in immunostaining?
I got a protocol for immunostaining specific for 3D culture, and according to the protocol, I should use with PBS 1X-Glycine 50mM to wash after 4% paraformaldehyde fixation?
I just have no idea what the function of Glycine here.
Anyone can help me with that?
Re: What is the funcion of Glycine in immunostaining?
When you cross-link with your formaldehyde mixture, you do it for a specific amount of time. The glycine then acts as a quencher for the cross-linking, assuring that you do not over cross-link your sample, which could lead to non-specific cross-linking.
Put simply, you stop the cross-linking reaction by quenching the reaction with glycine.
|funcion , glycine , immunostaining|
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