How do you save a Nitrocellulose WBlot?

spoler · #1 (**permalink**) 02-25-2009, 11:47 PM

Hi, Just interested if anyone has a nifty way to save a developed (TMBM) westen blot on Nitrocellulose (usually mini-blots by invitrogen). Currently in our lab we are cutting the excess blot away and making a sandwich between two clear sticky 96well plate-sealers. Any other ways to save the blot for archiving? (We also scan all the blots to a computer for future use as well).

Thanks for your replies!

gulg_genetic · #2 (**permalink**) 07-17-2009, 12:03 AM

You mean the membrane? I do keep them in a box at -20C. The blots, I would recommend thay you label with your name and date, and keep them in a folder..

wangleipurdue · #3 (**permalink**) 08-26-2009, 09:52 PM

I keep Nitrocellulose in 1X TBT-T at 4 C. It can be hold for 3 months (that is the longest time I tried).

wnym7247 · #4 (**permalink**) 09-27-2009, 06:50 AM

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How do you save a Nitrocellulose WBlot?

How do you save a Nitrocellulose WBlot? - Basic Lab Protocols and Techniques

How do you save a Nitrocellulose WBlot? -

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