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01-28-2009, 08:01 AM
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 bradford assay of plant protein I have a crude extract (plant protein) and i want to do bradford assay but i don't know how to proceed with it. The protocol i have says that i dilute my standard (bovine gamma globulin, BGG) and the crude protein extract in 0.15 NaCl. others however use water or buffer. So which should I use? I extracted the plant protein using pvpp, mops, dtt, edta mgcl2 and pmsf. do i have to use the same buffer to dilute standard and sample for bradford?  |
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| Tags |
| assay , bradford , plant , plant protein , protein |
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