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Staining Of Tapeworms

Staining Of Tapeworms - Basic Lab Protocols and Techniques

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Old 10-30-2006, 11:27 AM
Pipette Filler
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Default Staining Of Tapeworms



Anybody here knows some internet sites which consist some informations about staining of tapeworms?

We have some old methods with HE staining in our lab, but these are some old methods without details - i mean there is entire method step by step however we don't have for example proper time schedule regarding how long the segment of tapeworm should be hold in alcohol to be dehydrated.
These our methods were written by one doctor who did many
attempts when he was working here with all these parasites and he made many nice preparations for students. There is lack of descriptions on the internet about stainig methods of tapeworms, but maybe YOU have some informations and you can share with me.
Sometimes the tapeworms are very nice after staining and some time there are very bad - and not every time we know why...
And because of lack of material in these days it could be good to know how to do it properly ( staining ), bacuse we don't want to waste our precious tapeworms

And do you know maybe from what depends the quality of stained tapeworm.
Is it true that dry material won't be good after staining ?? and we won't see the internal structures after that ? (if the material will be dry ).
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Old 10-31-2006, 06:23 AM
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Default Re: Staining Of Tapeworms

Wow... different project!

I would try to standardize your treatments. Keep steps constant. Try different stains and pre-staining procedures.

Try at least a few worms with each, then pick the best one from the different treatments.
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Old 03-18-2011, 03:02 PM
Pipette Filler
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Default Re: Staining Of Tapeworms

hh, piease tell me the simplest procedure for tapeworm staining> thank you
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