Storing Samples

molecule2005 · #1 (**permalink**) 10-19-2006, 10:46 PM

Any ideas on the storage of samples?

How long can lysates stay in the -20C or -80C?

What about DNA can it stay long in the 4C

and RNA??? -80C?

thank you in advance for this

stefulj · #2 (**permalink**) 10-20-2006, 07:14 AM

Lysates -80, six months at least (didn´t test for longer time).
Rna -80,one year
Dna - for daily use 4, for longer time storage -20.

molecule2005 · #3 (**permalink**) 10-21-2006, 08:47 PM

Wow,
great information thanks stefulj!!

tuttu · #4 (**permalink**) 12-02-2006, 05:18 PM

Hi,

If its bacterial lysates then it colud be pelleted in Tris(10mM) and washed twice and resuspended in Tris+Glycerol mix and stored in -80 for a year, it worked for me. I used it for protein extraction!!.

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Storing Samples

Basic Lab Protocols and Techniques