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Storing Samples

Storing Samples - Basic Lab Protocols and Techniques

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  #1  
Old 10-19-2006, 10:46 PM
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Question Storing Samples



Any ideas on the storage of samples?

How long can lysates stay in the -20C or -80C?

What about DNA can it stay long in the 4C


and RNA??? -80C?

thank you in advance for this
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Old 10-20-2006, 07:14 AM
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Default Re: Storing Samples

Lysates -80, six months at least (didn´t test for longer time).
Rna -80,one year
Dna - for daily use 4, for longer time storage -20.
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Old 10-21-2006, 08:47 PM
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Default Re: Storing Samples

Wow,
great information thanks stefulj!!

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Old 12-02-2006, 06:18 PM
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Default Re: Storing Samples

Hi,

If its bacterial lysates then it colud be pelleted in Tris(10mM) and washed twice and resuspended in Tris+Glycerol mix and stored in -80 for a year, it worked for me. I used it for protein extraction!!.
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