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Plasmid isolation from Aeromonas

Plasmid isolation from Aeromonas - Basic Lab Protocols and Techniques

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Old 08-19-2008, 10:42 AM
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Question Plasmid isolation from Aeromonas



Hello Everyone,
i'm new to this forum.
I 'm working on Aeromonas isolates from food.
I have been trying to get plasmid from Aeromonas. I have tried isolation using Sigma and Roche plasmid isolation kits.
But whenever i'm isolating, i'm getting DNA of same size of around 20 kb in all the isolates.
May anyone help me in suggesting where i'm making the mistake.
i'm not able to understand How to differentiate between genomic and plasmid DNA.
How can i make it sure that it is plasmid DNa only?
is there any other method by which i can try to isolate it.

Expecting a prompt reply.

Regards,
Vandan
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aeromonas , isolation , plasmid


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