Originally Posted by kiki06
How can one remove Ethidium Bromide? Is there an easy way to clean it up?
Also how does one dispose of ethidium bromide?
Ethidium bromide not a carcinogen in first place....Here i passing you a article which i read long back"
"These in vitro tests, which comprise the entire body of evidence upon which the ethidium bromide hysteria is built, donít provide any evidence that ethidium bromide can exert a genotoxic effect in anything more complicated than a single cell or an unprotected embryo. In fact there is no direct evidence implicating ethidium bromide as a carcinogen in any animal.
For many years, ethidium bromide has been routinely administered for the treatment of African Sleeping Sickness in cattle. For this purpose, ethidium bromide is administered via subcutaneous or intramuscular injection with no reported increase in incidence of tumor formation or birth defects in the treated cattle. This suggests that ethidium bromide is far less genotoxic to animal systems than is presumed from the in vitro data.
The recommended, apparently non-toxic, dose of ethidium bromide is 1mg/kg of body weight in cattle. In comparison to this, the standard concentration used in molecular biology (around 1 microgram/litre), is low. Rosie Redfield puts it into perspective:
A 50kg researcher would need to drink 50,000 liters of gel-staining solution to get even the non-toxic dose used in cattle.
From this, the risks posed to a scientist handling a very weak solution of ethidium bromide, with a gloved hand (remember the cattle are injected with the stuff) are put into perspective.
A real concern is that the irrational and ill-informed fear of ethidium bromide drives us to solutions that are more dangerous than ethidium bromide itself.