Removal of Ethidium Bromide - Disposal of Ethidium Bromide

[kiki06]

How can one remove Ethidium Bromide? Is there an easy way to clean it up?

Also how does one dispose of ethidium bromide?

thanks

Kiki

[aftabac]

Hi Kiki
very good question. In most of the laboratories ethidium bromide is just put into the sink without any consideration, and we also know that it is strong mutagenic agent that can lead to many consequencies.
following is very good link regarding ethidium bromide disposal,
i hope it will be informative for all

http://web.princeton.edu/sites/ehs/chemwaste/etbr.html

regards
aftab

[aftabac]

Hey Kiki
there is another protocol for ethidium bromide removal given below
All solutions containing ethidium bromide must be decontaminated before being discarded.

1. Add 100 mg activated charcoal/ ml dye solution.

2. Let stand at least 1 hour with occasional stirring.

3. Filter through Whatman No.1 filter paper.

4. Pour the clear filtrate into the sink.

5. Place the filter paper containing the dye/charcoal mixture into the biohazard waste.


Regards
Aftab

[kiki06]

Thank you Aftab

[MARGARITA MARTINEZ]

Aftab .. Will Use Solution Of Activated Charcoal For Descontaminated Surface ???

Thank You

Wait For You Reply

Magui

[aftabac]

Well for surfaces, if there is spill of ethidium bromide solution, then i think first you should clear it with ethanol, and then that solution must be treated in a way i have given above to remove ethidium bromide, as otherwise it will be hard to use activated charcoal to clean the ethidium bromide conteminated surfaces.
but there is need of some more comments

best regards
aftab

[MARGARITA MARTINEZ]

Originally Posted by aftabac

Well for surfaces, if there is spill of ethidium bromide solution, then i think first you should clear it with ethanol, and then that solution must be treated in a way i have given above to remove ethidium bromide, as otherwise it will be hard to use activated charcoal to clean the ethidium bromide conteminated surfaces.
but there is need of some more comments

best regards
aftab

Aftab

Thank you for reply

Nice day for you

Margarita

[aftabac]

Thanks a lot Margarita
you are most welcome for discussion

best of luck for your reseach work

best regards
aftab ahmad

[megha]

hi,
i m a ph. d. student, working in the field of plant biotechnology and plant tissue culture..
want to be a part of these discussions

megha
INDIA