| | Re: Protein precipitation and overdried proteins
In best case scenario the overdried pellet can still be resuspended by adding time, sonication, gentle grinding, or using a stronger resuspension buffer. Worst case scenario you'll lose the overdried proteins and they won't resuspend.
Sometimes you need to have complimentary protein extraction/isolation methods for proteins based on their chemical characteristics: hydrophobic/hydrophilic proteins, glycoproteins, large mass proteins, high abundance/low abundance etc.
There isn't a perfect method for isolating 100% of proteins, you have to tailor your extraction/isolation to suit your target proteins.