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Nuclear Preps from Adipose tisue

Basic Lab Protocols and Techniques



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Old 04-07-2008, 12:46 PM
Pipette Filler
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Default Nuclear Preps from Adipose tisue

Hi

I was hoping someone might be able to help me with some tips on preparing nuclei, or a nuclear enriched pellet from rodent brown adipose tissue. We have tried adapting our existing cell fractionation techniques, but this has failed to give a good yield of nuclear material.

Thnaks in advance

Stuart
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