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ELISA Practical

ELISA Practical - Basic Lab Protocols and Techniques

ELISA Practical -


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  #1  
Old 02-25-2008, 10:29 PM
Pipette Filler
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Default ELISA Practical



Hi there,
Can anyone please tell me the reason for using MArvel milk powder with PBS-Tween to wash the plate. And why would you use two different concentrations e.g 3% MArvel in PBS-Tween and then 0.1% Marvel in PBS-Tween?

Thanks in advance for any help
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Old 08-05-2008, 04:42 PM
Pipette Filler
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Default Re: ELISA Practical

The milk powder contains proteins that act as a blocking agent to block nonspecific residues from interacting with the ELISA plate. The second lower concentratino of milk powder is probably to equilibrate the plate to a similar buffer as what will be used in the subsequent step.
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