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ELISA Practical - Basic Lab Protocols and Techniques
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Can anyone please tell me the reason for using MArvel milk powder with PBS-Tween to wash the plate. And why would you use two different concentrations e.g 3% MArvel in PBS-Tween and then 0.1% Marvel in PBS-Tween?
Thanks in advance for any help
Re: ELISA Practical
The milk powder contains proteins that act as a blocking agent to block nonspecific residues from interacting with the ELISA plate. The second lower concentratino of milk powder is probably to equilibrate the plate to a similar buffer as what will be used in the subsequent step.
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