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slot blot - Basic Lab Protocols and Techniques

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Old 02-07-2008, 08:22 PM
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Default slot blot



I think I have made a pMAL fusion with my protein. I need to identify with antibody, but have only ever done SDS-PAGE Western blotting. Boss says slot blot and densitometry are easier and faster. I would like to learn some basics about this, but Google is just giving me refs to papers wherein the investigators used slot blot -- no basic info. Can anyone point me in the right direction? We use horseradish perox. for Westerns.
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Old 02-22-2008, 09:13 PM
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Default Re: slot blot

Try searching apparatus manuals for slot blots like "biorad slot blot" on google.
Here are two results:
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Old 02-25-2008, 05:03 PM
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Default Re: slot blot

Thank you danfive. I have checked some out.
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